What is the delivery schedule for a contract production?
The delivery of the purified antibodies from a standard CL1000 flask production run is 8-10 weeks after
signing of the contract. This entails 1-2 weeks of expansion of cells and establishment of the CL1000 flask,
5 weeks of production and harvesting and 1-2 weeks down-stream processing and QC. If a larger amount is
required, the CL1000 production can be continued for an additional 1-5 weeks or several flasks can be run
in parallel.
For large-scale productions bimonthly deliveries will be the best option, giving first delivery 8-10 weeks
after the production run is started. Read more.
How much antibody can I expect from one standard CL1000 production?
The yield depends on the productivity of the clone. The concentration of antibodies in the harvest supernatant
is approximately 50 times the concentration in a cell culture supernatant, and a standard production run
gives approximately 100-120mL harvest supernatant. The antibody is purified using a suitable purification
resin according to isotype and origin of the antibody, to optimise the final yield.
Read more.
My hybridoma is growing in a serum free medium, do you do productions using serum
free medium? Diatec Monoclonals standard medium is a specially adapted RPMI1640 with additional L-Glutamine and glucose,
added foetal bovine serum of FDA approved origin. However, we have had several successful productions using
different serum free media on customers’ request.
I have an antigen against which I would like to establish an antibody.
Can Diatec Monoclonals help me? Diatec Monoclonals has several years of experience in establishing hybridomas
from customers’ antigens. Our hybridoma production projects are tailored to suit each customer (e.g. mouse
strain, number of immunisations etc.) and our programs are modular so the customer can choose how extensive
each phase of the program should be.
Read more.
I have a hybridoma from which I would like to produce monoclonal antibodies,
but I do not know anything about it regarding productivity, subclass etc. Can Diatec Monoclonals help?
Diatec Monoclonals AS has well established systems for analysis for the most crucial parameters that ensure optimal
production. On request we can perform analysis of antibody productivity of cell culture supernatant,
determine the percentage of producing cells, subclass and specificity analysis and other assays. Full
certificates of analysis are supplied.
I need a small amount of antibody now for development purposes, with a potential
scaling-up at a later date.
Diatec Monoclonals in vitro production systems are adaptable to suit different production scales. The Integra Celline
CL1000 production scale will cover your initial research needs. The use of our in vitro production technology
will ensure that the products delivered from this production scale will be of the same high, stable quality
as products in larger scale systems (Maximizer) when you are ready to scale up.
Read more.
Do you perform custom conjugations of antibodies to fluorochromes and enzymes?
Different conjugation methods are one of Diatec Monoclonals special fields of expertise. We have extensive experience
in conjugating antibodies to fluorochromes and enzymes, among them FITC/fluorescein, phycoerythrin (PE),
allophycocyanin (APC), biotin, horse radish peroxidase (HRP) and alkaline phosphatase (ALP).
General
Production
All our monoclonal antibodies are produced
in vitro, yielding antibodies
with a higher and more consistent quality than ascites
production in mice.
Orders and delivery
Orders are placed by contacting us directly.
Quality control
Our quality system is established according to ISO 9001:2000. We were first accredited 12 February 1998. For questions about quality assurance
of our products and services, contact Øyvind Røe oyvind.roe@diatec.com.
Services
Contract production of monoclonal antibodies, development
of new clones, etc.
Why are all productions at Diatec Monoclonals performed in vitro?
After Köhler and Milstein's discovery of hybridomas producing monoclonal antibodies in 1975, production was
initially done in the ascitic fluid of production animals. Hollow fibre technology was developed over the
same period, and has over the last decade given us an excellent alternative to using production animals.
Due to animal welfare concerns, and the possibility of obtaining a pure product, without animal
contaminants, with high batch to batch reproducibility, hollow fibre technology has proved to be a very
reasonable choice. Read more.
Quality control at Diatec Monoclonals AS
Diatec Monoclonals is certified according to the ISO9001:2000 standard.
All products and services delivered from Diatec Monoclonals are according to this standard. Our procedures are
documented through control plans, standard operation procedures and full historical records of procedures,
ensuring full traceability and documentation of our products? high quality.
What are the advantages of using monoclonal human IgE compared to IgE from a donor patient?
Diatec Monoclonals Human IgE is unique in that it is produced
in vitro from a hybridoma. This unique source guarantees freedom from contamination by other immunoglobulin
isotypes. The original IgE producing cell was obtained from a healthy donor tested negative by US-FDA
approved tests for HIV, HCV and Hepatitis B. The purity of the product is >98%, tested by SDS-PAGE.
This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch v
ariation. Other applications include immunochemistry and cellular immunology research. In contrast to IgE
from a myeloma patient, where batch-to-batch variability may be high, the monoclonal source of Diatec IgE
ensures very high reproducibility.
Does the product no. 5010, anti-human C5b-9 antibody bind both the soluble and
membrane bound form of the TCC complex?
This antibody recognises C5b-9, the terminal complement complex (TCC) and is directed against a neoepitope
exposed in C9 when incorporated into the terminal complement complex (TCC). The clone aE11 produces
monoclonal antibodies against neoantigens of both membrane bound (MAC) and fluid phase (SC5b-9) complexes.
Read more.
Is it possible to order bulk amounts of your list products?
Should you require a larger amount (from 10 to several hundred milligrams) of our unconjugated products,
please contact us for a quote. We can offer substantial discounts
to our list prices on bulk purchases, and can adapt the concentration, buffer etc. to suit your application.
Read more.
I would like to test an antibody in my own system. Can I have a test sample
of your antibodies?
All of our conjugated products are available in 10-test aliquots for you to test for performance in and
compatibility with your assays and systems.
What is the difference between the two anti-human CD3 clones you have?
Diatec Monoclonals AS stocks two different clones against the leukocyte marker CD3,
UCHT-1 and
CRIS-7.
The antibody from clone UCHT-1 is your best choice for applications such as frozen tissue sections,
histology, RIA, immunoprecipitation and flow cytometry, whereas the CRIS-7 product is well suited for
purposes such as stimulation of T-cells. It is observed that the epitope for CRIS-7 antibody may be blocked
or denatured by fixation.
Should I order with or without azide?
Sodium azide (NaN3) is added to our products as a preservative at a concentration of 0.09%. If your
application involves live cells or enzymes sensitive to sodium azide (e.g. horse radish peroxidase) you
should order an azide free product. Azide free products are denoted by ?a at the end of the product number.
How are my products shipped?
Products from Diatec Monoclonals AS are shipped by FedEx.
This ensures efficient, door-to-door delivery of your order.
Should you wish to have your product shipped by a different courier company, we will be happy to arrange
this, but reserve the right to ship EXW Oslo. See list of delivery costs.
Is there a local distributor near me?
Diatec Monoclonals AS has a network of distributors around the world, if you prefer dealing directly with your
local representative. Go to distributor contact details.
Where can I obtain an MSDS for Diatec Monoclonals AS products?
Please contact us by e-mail diatec@diatec.com, and we will be happy to send you an MSDS.
